Journal: International Journal of Medical Sciences

Article Title: Ginsenoside Rg1 alleviates acute liver injury through the induction of autophagy and suppressing NF-κB/NLRP3 inflammasome signaling pathway

doi: 10.7150/ijms.50919

Figure Lengend Snippet: G-Rg1 suppressed apoptosis in mice with CCl 4 -induced acute liver injury through autophagic induction. (A) Apoptotic cells were stained with a TUNEL apoptosis detection kit in control group, G-Rg1(4 mg/ml) control group, G-Rg1(4 mg/ml) + RPA(1 mg/kg) group and G-Rg1(4 mg/ml) + 3-MA(1 mg/kg) group at 24 h after the CCl 4 injection. All of the images were obtained on an inverted fluorescence microscope. (B) Flow cytometry was also performed to detect the apoptotic hepatocytes (n=3). *P<0.05.

Article Snippet: All images were obtained with an inverted fluorescence microscope (Nikon Eclipse E800, Tokyo, Japan).

Techniques: Staining, TUNEL Assay, Control, Injection, Fluorescence, Microscopy, Flow Cytometry

Journal: BMC Research Notes

Article Title: FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis

doi: 10.1186/s13104-018-3763-1

Figure Lengend Snippet: FANCC and UNC5A co-localize to neurite outgrowth. a , b Representative microscopic images of SH-SY5Y cells incubated with RA (10 μM) for 6 days prior to analysis. Differentiated SH-SY5Y cells were labeled with antibodies against FANCC (green) and UNC5A (red). c SH-SY5Y cells were incubated with recombinant Netrin-1 (500 ng/ml) prior to analysis. Labeled cells were visualized by confocal fluorescence microscopy at ×60 and ×100 magnification using a Nikon E800 microscope equipped with a C1 confocal system

Article Snippet: Images were acquired using a Nikon E800 fluorescent microscope equipped with a C1 confocal system (Nikon Canada) at 100× magnification.

Techniques: Incubation, Labeling, Recombinant, Fluorescence, Microscopy

Journal: BMC Research Notes

Article Title: FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis

doi: 10.1186/s13104-018-3763-1

Figure Lengend Snippet: FANCC and UNC5A are required for neurite outgrowth. a Representative microscopic images of SH-SY5Y cells stably transduced with shRNA against FANCC (FANCCi), UNC5A (UNC5Ai), or control noncoding scrambled shRNA (Control) vectors (upper panels) and following differentiation with retinoic acid (+RA at 10μM; lower panels). Cells were visualized at ×40 magnification. b Western blots showing the depletion of FANCC using either one shRNA (sh-C1) or a mixture of 3 (sh-C3) against FANCC (upper blot) and a mixture of 5 shRNA against UNC5A (sh-U; lower blot) in SH-SY5Y cells. c Control cells transfected with empty vectors; NT: untransfected cells. c UNC5A - and FANCC -depleted SH-SY5Y cells (UNC5Ai and FANCCi, respectively) induced to differentiate with RA (10μM) were labeled with anti-UNC5A (blue) and anti-FANCC (green) antibodies and phalloidin conjugates (red). The labeled cells were visualized by confocal fluorescence microscopy at ×100 magnification using a Nikon E800 microscope equipped with a C1 confocal system. d Estimated neurite length measured estimated Control (n = 15) FANCCi (n = 21) and UNC5Ai (n = 11) ***p > 0.0001

Article Snippet: Images were acquired using a Nikon E800 fluorescent microscope equipped with a C1 confocal system (Nikon Canada) at 100× magnification.

Techniques: Stable Transfection, Transduction, shRNA, Western Blot, Transfection, Labeling, Fluorescence, Microscopy